Journal: Biomedicines

Article Title: Assessment of the Polyphenolic Profile and Beneficial Effects of Red and Green Propolis in Skin Inflammatory Conditions and Oxidative Stress

doi: 10.3390/biomedicines13092229

Figure Lengend Snippet: Representative confocal micrographs of the fluorescent immunostaining of HIF-1α (red) to assess the effect of Brazilian propolis on HIF-1α stabilization and nuclear translocation in HaCaT cells after 6 h of treatment (magnification 60×, 50 μm scale). Nuclei were stained with DAPI (blue), whereas F-actin was stained with TRITC-phalloidin (orange).

Article Snippet: Finally, cells were washed 3 times with PBS, mounted with coverslips using a drop of ProLong Gold antifade reagent with DAPI (Cell Signaling Technology B.V., Leiden, The Netherlands), and imaged with a confocal laser scanning microscope (LSM 900, Carl Zeiss S.p.a., Milan, Italy).

Techniques: Immunostaining, Translocation Assay, Staining

Journal: Biomedicines

Article Title: Assessment of the Polyphenolic Profile and Beneficial Effects of Red and Green Propolis in Skin Inflammatory Conditions and Oxidative Stress

doi: 10.3390/biomedicines13092229

Figure Lengend Snippet: Representative confocal micrographs of the fluorescent immunostaining of HIF-1α (red) to assess the effect of Brazilian propolis on HIF-1α stabilization and nuclear translocation in HaCaT cells after 24 h of treatment (magnification 60×, 50 μm scale). Nuclei were stained with DAPI (blue), whereas F-actin was stained with TRITC-phalloidin (orange).

Article Snippet: Finally, cells were washed 3 times with PBS, mounted with coverslips using a drop of ProLong Gold antifade reagent with DAPI (Cell Signaling Technology B.V., Leiden, The Netherlands), and imaged with a confocal laser scanning microscope (LSM 900, Carl Zeiss S.p.a., Milan, Italy).

Techniques: Immunostaining, Translocation Assay, Staining

Journal: Biomedicines

Article Title: Assessment of the Polyphenolic Profile and Beneficial Effects of Red and Green Propolis in Skin Inflammatory Conditions and Oxidative Stress

doi: 10.3390/biomedicines13092229

Figure Lengend Snippet: Representative confocal micrographs of the fluorescent immunostaining of HIF-1α (red) to assess the effect of Brazilian propolis on HIF-1α stabilization and nuclear translocation in HDF cells after 6 h of treatment (magnification 60×, 50 μm scale). Nuclei were stained with DAPI (blue), whereas F-actin was stained with TRITC-phalloidin (orange).

Article Snippet: Finally, cells were washed 3 times with PBS, mounted with coverslips using a drop of ProLong Gold antifade reagent with DAPI (Cell Signaling Technology B.V., Leiden, The Netherlands), and imaged with a confocal laser scanning microscope (LSM 900, Carl Zeiss S.p.a., Milan, Italy).

Techniques: Immunostaining, Translocation Assay, Staining

Journal: Biomedicines

Article Title: Assessment of the Polyphenolic Profile and Beneficial Effects of Red and Green Propolis in Skin Inflammatory Conditions and Oxidative Stress

doi: 10.3390/biomedicines13092229

Figure Lengend Snippet: Representative confocal micrographs of the fluorescent immunostaining of HIF-1α (red) to assess the effect of Brazilian propolis on HIF-1α stabilization and nuclear translocation in HDF cells after 24 h of treatment (magnification 60×, 50 μm scale). Nuclei were stained with DAPI (blue), whereas F-actin was stained with TRITC-phalloidin (orange).

Article Snippet: Finally, cells were washed 3 times with PBS, mounted with coverslips using a drop of ProLong Gold antifade reagent with DAPI (Cell Signaling Technology B.V., Leiden, The Netherlands), and imaged with a confocal laser scanning microscope (LSM 900, Carl Zeiss S.p.a., Milan, Italy).

Techniques: Immunostaining, Translocation Assay, Staining